Immunoelectrophoresis - urine

Alternative names:

gammaglobulin electrophoresis - urine; IEP - urine; immunoglobulin electrophoresis - urine; urine immunoglobulin electrophoresis

How the test is performed:

Child or adult:
Collect a "clean-catch" ("midstream") urine sample. To obtain a clean-catch sample, men or boys should wipe clean the head of the penis. Women or girls need to wash the area between the lips of the vagina with soapy water and rinse well. As you start to urinate, allow a small amount to fall into the toilet bowl (this clears the urethra of contaminants). Then, in a clean container, catch about 1 to 2 ounces of urine and remove the container from the urine stream. Give the container to the health care provider or assistant.

Infant:
Thoroughly wash the area around the urethra. Open a urine collection bag (a plastic bag with an adhesive paper on one end), and place it on your infant. For males, the entire penis can be placed in the bag and the adhesive attached to the skin. For females, the bag is placed over the labia. Place a diaper over the infant (bag and all). Check your baby frequently and remove the bag after the infant has urinated into it. For active infants, this procedure may take a couple of attempts--lively infants can displace the bag, causing an inability to obtain the specimen. The urine is drained into a container for transport back to the health care provider.

Immunoelectrophoresis is a laboratory technique. Electrical charges are used to separate and identify the various immunoglobulins.

It uses a combination of protein electrophoresis and an antigen-antibody interaction. Protein electrophoresis indicates the presence of immunoglobulins as a group. Immunoelectrophoresis enhances the ability to identify the specific immunoglobulins through the use of antibodies that only react with the proteins of interest.

Specific lab technique: Monospecific (that is, specific for only one antigen) antiserum is overlaid on the zone of the electrophoretogram (the paper graph used with protein electrophoresis), which contains the unidentified protein. The presence of a precipitin band indicates that the antigen is present for the monospecific antiserum used.

How to prepare for the test:

Collection of the first morning urine, which is the most concentrated, may be recommended.

If the collection is being taken from an infant, a couple of extra collection bags may be necessary.

How the test will feel:

The test involves only normal urination, and there is no discomfort.

Why the test is performed:

This test is used to roughly measure the amounts of various immunoglobulins in urine. Most often, this is used as a screening test, particularly in people who have protein in the urine (demonstrated on urinalysis or other test) when urine protein electrophoresis indicates a significant amount of globulin proteins (antibodies).