CSF smear
How the test is performed:
The CSF is usually obtained through a lumbar puncture (spinal tap).
A small amount of the CSF is removed and sent to the lab. A smear involves spreading a thin sample on a glass slide, fixing (preserving) the sample, and staining. It is then examined under a microscope.
How to prepare for the test:
There is no special preparation other than that for CSF collection.
You must sign a consent form. You must be prepared to remain in the hospital for at least the 6 to 8 hours that you must remain flat.
Infants and children:
The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experience, and level of trust. For specific information regarding how you can prepare your child, see the following topics as they correspond to your child's age:
How the test will feel:
The position may be uncomfortable, but it is imperative that the person remain in the curled position to avoid moving the needle and possibly injuring the spinal cord.
The scrub will feel cold and wet. The anesthetic will sting or burn when first injected. There will be a hard pressure sensation when the needle is inserted, and there is usually some brief pain when the needle goes through the meninges. This pain should stop in a few seconds. Overall, discomfort is minimal to moderate. The entire procedure usually takes about 30 minutes but may take longer. The actual pressure measurements and fluid collection only takes a few minutes.
Why the test is performed:
The examination under the microscope checks for bacteria.
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