Fluorescein eye stain

Alternative names:

eye test - fluorescein

How the test is performed:

Dye is placed in the eye, using an "eye dropper" or blotting paper, and allowed to cover the surface of the cornea. The individual is asked to blink to move the dye. A cobalt blue light is then directed on the eye, causing the fluorescein to glow green. Abnormalities in the corneal epithelium will cause the dye to stain that region.

The ophthalmologist or optometrist (eye doctor) can determine the location and probable cause of the cornea problem depending on the size, location, and shape of the staining.

How to prepare for the test:

If contact lenses are worn, they will need to be removed before the test.

Infants and children:
The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experience, and level of trust. For specific information regarding how you can prepare your child, see the following topics as they correspond to your child's age:

• infant test or procedure preparation (birth to 1 year)
• toddler test or procedure preparation (1 to 3 years)
• preschooler test or procedure preparation (3 to 6 years)
• schoolage test or procedure preparation (6 to 12 years)
• adolescent test or procedure preparation (12 to 18 years)

How the test will feel:

If eyes are extremely dry, the blotting paper may be slightly scratchy. The dye feels like liquid in the eye and should not cause itching or other discomfort.

Why the test is performed:

This test is useful in determining if there is a scratch or other problem with the surface of the cornea. It can also be used to detect foreign bodies on the eye surface, and determine if there is an injury to the eye or eye infection. It can be used after contacts are prescribed to determine if there is adequate tear exchange between the cornea and the tear film.